| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101129 |
| Chromosome: | chromosome 3 |
| Location: | 2444357 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159300 | (1 of 2) PF05255 - Uncharacterised protein family (UPF0220) (UPF0220) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAAGCGGCCTGGCAGTACGGAACGTCA |
| Internal bar code: | TCCGCCGACGGGTCTTTTCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 816 |
| LEAP-Seq percent confirming: | 90.0627 |
| LEAP-Seq n confirming: | 2873 |
| LEAP-Seq n nonconfirming: | 317 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGTAGGGGACATGAAGG |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |