Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.101134 |
Chromosome: | chromosome_4 |
Location: | 2035680 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g219000 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTTGCGAGTGCCCTACATGTTCTTATTTT |
Internal bar code: | TCGTTGCTAGGGTCTGCCGATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 447 |
LEAP-Seq percent confirming: | 97.6471 |
LEAP-Seq n confirming: | 83 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTATTGCCAAACGCACAAT |
Suggested primer 2: | TAGTTTGAGCATGTGCGAGG |