| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101140 |
| Chromosome: | chromosome 3 |
| Location: | 5134115 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181900 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACGCACAATGTCAAGTCGCAAGAAAGA |
| Internal bar code: | CGATCGACCAAAACCGTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.0753 |
| LEAP-Seq n confirming: | 2893 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACGGTATGCATCTGCCC |
| Suggested primer 2: | CCACTCGTGGTGAGATGATG |