| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101143 |
| Chromosome: | chromosome 1 |
| Location: | 218797 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001400 | ZMP1 | (1 of 1) 3.4.24.36 - Leishmanolysin / Promastigote surface endopeptidase; Zinc-metallopeptidase-like protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGCGGCCCACCTTGCTAGTATGAGCCG |
| Internal bar code: | TACTTAGCTTGAGGTCGGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 174 |
| LEAP-Seq percent confirming: | 98.7047 |
| LEAP-Seq n confirming: | 381 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGGCAGACGAAGCGAAG |
| Suggested primer 2: | CCCATTGGGACCTATTCCTT |