Insertion junction: LMJ.RY0402.101200_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.101200
Chromosome:chromosome 1
Location:3621635
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g023350 THO2 Oligoendopeptidase; (1 of 1) 3.4.24.16 - Neurolysin / Neurotensin endopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTACCACGGATACGGTGAGGGGGCGGAGG
Internal bar code:AGACGCGTAGACCGCGCTTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:24.3243
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAGCCCAAACTAAACCG
Suggested primer 2:CTGAACTCTGCACCCTCCTC