| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101220 |
| Chromosome: | chromosome 13 |
| Location: | 150535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563150 | PAB1,CGLD8 | Assembly factor for CF1 or F1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGTACCACGTGGCGTGCGTCTGCTCG |
| Internal bar code: | CTGGAGGCTTATTAGGCCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 98.4619 |
| LEAP-Seq n confirming: | 4865 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAGGCCCACCTGTTAGG |
| Suggested primer 2: | TCCACTTAATACCCCCTCCC |