| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101246 |
| Chromosome: | chromosome 14 |
| Location: | 3411859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630550 | DEG1B,DEG13,DEG12 | Deg protease; (1 of 3) PTHR22939//PTHR22939:SF81 - SERINE PROTEASE FAMILY S1C HTRA-RELATED // PROTEASE DO-LIKE 1, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACAACCCCGACCCCTTCCCCCCCCACAC |
| Internal bar code: | AAAACGATCGACGCCTTAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 97 |
| LEAP-Seq percent confirming: | 94.5455 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAAGTAGCAGGGCTCCA |
| Suggested primer 2: | CCCAGTGTGGTCAACATCAG |