| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101265 |
| Chromosome: | chromosome_3 |
| Location: | 5073357 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g181100 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAAGAACATGCCGCTGCGGCTCTGGCGCA |
| Internal bar code: | CGTCCAGACGCGCCTTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 98.8142 |
| LEAP-Seq n confirming: | 250 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGACCAGCATCAGCAC |
| Suggested primer 2: | AACGGCAGGGGCTATAACTT |