Insertion junction: LMJ.RY0402.101267_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.101267
Chromosome:chromosome 13
Location:781128
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g566900 (1 of 1) PTHR11289:SF0 - BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTCCAAAGGTCCAGGTCGACACACCCGC
Internal bar code:TTTTCCTCTCTGTCTCCTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:126
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:127
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACTCCATGTCACACAACCC
Suggested primer 2:CCAGGTCTCGGTACCTGTGT