| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101270 |
| Chromosome: | chromosome 9 |
| Location: | 4242343 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394436 | (1 of 1) PF03030 - Inorganic H+ pyrophosphatase (H_PPase) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTCAACTGTCCTCATCAGGGTGGGCTAT |
| Internal bar code: | GTCGCCCTCTGTCCAACGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 94.2021 |
| LEAP-Seq n confirming: | 1771 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCTGGAAGGCTTTGCT |
| Suggested primer 2: | GTATAGGCACTGCCCGTCAT |