Insertion junction: LMJ.RY0402.101282_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.101282
Chromosome:chromosome 9
Location:7495557
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g413900 GT90F18,GT90-18 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 18 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGAAGCCCAAGCCCGAGCACGGAGACGCG
Internal bar code:CTAGAGGTCGTGTTACTCCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1146
LEAP-Seq percent confirming:99.6617
LEAP-Seq n confirming:2651
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAATCGAGCGCATCATAG
Suggested primer 2:CTGCTAGACCTGGAGAACGG