| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101337 |
| Chromosome: | chromosome 14 |
| Location: | 3404577 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630500 | TIE2 | (1 of 1) K15323 - tRNA-splicing endonuclease subunit Sen34 (TSEN34); tRNA-intron endonuclease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGTAAGATCACAGTCGTGACATGCA |
| Internal bar code: | AAATTACGTTGTGGCAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 97.7968 |
| LEAP-Seq n confirming: | 799 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATCCACTACCTGTCCT |
| Suggested primer 2: | TGTCCTACTCACACCACCCA |