| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101374 |
| Chromosome: | chromosome 4 |
| Location: | 117903 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216700 | PHOX,PHO5 | Phosphate-repressible alkaline phosphatase; (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAAACGATCACCACTGCTCACGACTGGC |
| Internal bar code: | GGCTGAGGTGTCTATTCAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 322 |
| LEAP-Seq percent confirming: | 99.6483 |
| LEAP-Seq n confirming: | 3117 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACCCAGCTTATGAACGC |
| Suggested primer 2: | GGACCAGCACTACCACCAGT |