| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101376 |
| Chromosome: | chromosome 12 |
| Location: | 3702603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514600 | (1 of 1) 2.7.4.27 - ([Pyruvate, phosphate dikinase] phosphate) phosphotransferase / Pyruvate, phosphate dikinase regulatory protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCAGCTGCTGGCGCCGGACGTGCACAC |
| Internal bar code: | GACAGCCTTCGGTCCCGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 764 |
| LEAP-Seq percent confirming: | 94.3215 |
| LEAP-Seq n confirming: | 5498 |
| LEAP-Seq n nonconfirming: | 331 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCTTGAGCAAACGTGAA |
| Suggested primer 2: | ATTACCCTTACCTTGCGCCT |