| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101417 |
| Chromosome: | chromosome 5 |
| Location: | 970267 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCCATCCCCCCCGGGACTGACCAACC |
| Internal bar code: | CACTAGGCGAGACTTCGGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 98.3681 |
| LEAP-Seq n confirming: | 1507 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCTCCCCTTCAACCTTC |
| Suggested primer 2: | TTGCGCACTTGTAGTTCACC |