| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101428 |
| Chromosome: | chromosome 3 |
| Location: | 2527394 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160000 | HDA3 | (1 of 3) PTHR10625//PTHR10625:SF102 - HISTONE DEACETYLASE // HISTONE DEACETYLASE 10; RPD3/HDA1 type histone deacetylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCCCCTCCTCCACCGCCTCCACCGCCCC |
| Internal bar code: | CTTGGAAGAGTATATACCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 96.5607 |
| LEAP-Seq n confirming: | 4464 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATCCAAACGTCACGTCAT |
| Suggested primer 2: | ACGACCACGGCACCTACTAC |