Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.101437 |
Chromosome: | chromosome 9 |
Location: | 5749483 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g401812 | (1 of 1) PF00580//PF13086//PF13087 - UvrD/REP helicase N-terminal domain (UvrD-helicase) // AAA domain (AAA_11) // AAA domain (AAA_12) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACACGCACAAGTTCAAGGAGCAGACGT |
Internal bar code: | GCAACCAGACGGGATGCAAGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 79 |
LEAP-Seq percent confirming: | 98.4375 |
LEAP-Seq n confirming: | 63 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGATGGCAATGGTGAACA |
Suggested primer 2: | CATACACTCCCCAACCTGCT |