| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101439 |
| Chromosome: | chromosome 12 |
| Location: | 1550569 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486900 | (1 of 1) 1.3.1.88 - tRNA-dihydrouridine(16/17) synthase (NAD(P)(+)) / tRNA-dihydrouridine synthase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGCTGCAGTACGCCGCGCCGCCTGGC |
| Internal bar code: | TTCCGAGAGTTGATCGGTTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 65.4354 |
| LEAP-Seq n confirming: | 744 |
| LEAP-Seq n nonconfirming: | 393 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGAGGGAGTGGTTGAAG |
| Suggested primer 2: | GAGGAAGGGGAAGTCGTTTC |