| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101454 |
| Chromosome: | chromosome 17 |
| Location: | 3430986 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAGGCCTTACCAAACCAACCCCAGGCTC |
| Internal bar code: | GAGAGGAGGTAAGAGCAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.0993 |
| LEAP-Seq n confirming: | 10452 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTCCTACGCTAAGCTC |
| Suggested primer 2: | GTCAGTAGTGGGTCGGCATT |