Insertion junction: LMJ.RY0402.101469_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.101469
Chromosome:chromosome 4
Location:1377655
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g214504 (1 of 1) IPR000095//IPR001190//IPR008979//IPR017448 - CRIB domain // SRCR domain // Galactose-binding domain-like // SRCR-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCCCGCCACCCCGCTCTCCTCCTCGCT
Internal bar code:CCTATTTGCCCTTCTTAAGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:491
LEAP-Seq percent confirming:99.1026
LEAP-Seq n confirming:773
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCTGGAGCGAGACGAATC
Suggested primer 2:CACACGATGTTGTCCGTTTC