Insertion junction: LMJ.RY0402.101503_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.101503
Chromosome:chromosome 6
Location:2245193
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g266416 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTTCTCGACGTCCACAGCCTCGCTCTC
Internal bar code:ATATGACTCCGGGCTCAGGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:758
LEAP-Seq percent confirming:86.1757
LEAP-Seq n confirming:2855
LEAP-Seq n nonconfirming:458
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTACGCGAACTCCACACC
Suggested primer 2:GGCGACTACTCAAGCAAAGG