| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101516 |
| Chromosome: | chromosome 1 |
| Location: | 6336304 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045200 | (1 of 1) 5.3.99.6 - Allene-oxide cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACAACGTCGCCGGCCTGCGGGGGCGAG |
| Internal bar code: | TGTTCAACAAGTGTCTAGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.1728 |
| LEAP-Seq n confirming: | 1079 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGAGTGGCGGTTATGTTG |
| Suggested primer 2: | GGCTCTGGCTATGGTAGCTG |