| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101534 |
| Chromosome: | chromosome_16 |
| Location: | 1629681 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g654300 | RSP23 | Radial spoke protein 23 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGAAGCCATTCAGCTCGATAAGCTGCATG |
| Internal bar code: | ATCGTGCCATCCACCAGTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 94.6396 |
| LEAP-Seq n confirming: | 512 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTACCACTTAGGCTGCTC |
| Suggested primer 2: | CACTATGCAGCTCTGGGTCA |