| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101543 |
| Chromosome: | chromosome 2 |
| Location: | 8492497 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141826 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGACGCAAGGCCCTCCTGCGCCAGGCAG |
| Internal bar code: | GTTTTTGACCCTCGTGTCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 99.7028 |
| LEAP-Seq n confirming: | 2684 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAAACCATCGGTGTGGGAT |
| Suggested primer 2: | AACATTGCGCAAAGACAGTG |