| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101543 |
| Chromosome: | chromosome 8 |
| Location: | 3351330 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374150 | (1 of 11) IPR011146 - HIT-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCGCTTCCCGCACCTTCATACATCATG |
| Internal bar code: | CGCCTAAGGGGAGGATATATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 501 |
| LEAP-Seq percent confirming: | 99.2322 |
| LEAP-Seq n confirming: | 517 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGAATTCCATTCCGAGT |
| Suggested primer 2: | CAAGGAAAACATAGCGCACA |