| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101565 |
| Chromosome: | chromosome 9 |
| Location: | 5124853 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398808 | (1 of 2) PF01465 - GRIP domain (GRIP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGCGAGGGATTGCAGGGCAGCCAAGCG |
| Internal bar code: | TCCGGAACTGGTTAATGATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 99.6154 |
| LEAP-Seq n confirming: | 777 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGTCATGTTGCTGATCT |
| Suggested primer 2: | TCTATCCACGGGGTAGCTTG |