| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101584 |
| Chromosome: | chromosome 8 |
| Location: | 4278596 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381050 | CEP8 | Cysteine endopeptidase; (1 of 3) 3.4.22.67 - Zingipain / Zingibain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCGTGGGGTTCCAGGCTTATCGCAGG |
| Internal bar code: | TTGTTCTCTCTTCGCCACAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 62.3563 |
| LEAP-Seq n confirming: | 1953 |
| LEAP-Seq n nonconfirming: | 1179 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGTGTGTGTGTTTTGCCC |
| Suggested primer 2: | CACGCCAGCAGTTATAAGCA |