| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101624 |
| Chromosome: | chromosome 5 |
| Location: | 1286379 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245100 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTATTCGTTGATCGCCTCCCAACTCCC |
| Internal bar code: | ATCGGGCGCGGCGTAACGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 98.1341 |
| LEAP-Seq n confirming: | 3629 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTTCATTCAGGTTGCGG |
| Suggested primer 2: | TGCTGCTGTGGTAAATAGCG |