| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101625 |
| Chromosome: | chromosome 16 |
| Location: | 4883806 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685901 | (1 of 1) K18423 - exportin-2 (importin alpha re-exporter) (CSE1, CAS, XPO2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAATTGCCCCGTAGCCAGGAAGTTGTA |
| Internal bar code: | CCCTCTCAGAGATGTGCATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.4583 |
| LEAP-Seq n confirming: | 3305 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTAAGTTTGGGCTGGTA |
| Suggested primer 2: | CTGGTTTCCATGCATCTCCT |