| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101634 |
| Chromosome: | chromosome 14 |
| Location: | 1723983 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619550 | POA7 | (1 of 1) K02727 - 20S proteasome subunit alpha 7 (PSMA3); 20S proteasome alpha subunit G | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGTTCCTAGCTCAGAGCACATCCGATTG |
| Internal bar code: | GGGTGTACACGAGATTCCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 98.7335 |
| LEAP-Seq n confirming: | 1793 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGTGCGATGGGACAGTG |
| Suggested primer 2: | CCTGTCTGCCCTCTGCTATC |