| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101653 |
| Chromosome: | chromosome 14 |
| Location: | 1710966 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACCCTTGCACTCCGCGGCTTGTGAAT |
| Internal bar code: | ACATAACTTGTGGGAGGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 585 |
| LEAP-Seq percent confirming: | 98.5287 |
| LEAP-Seq n confirming: | 8572 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGCGGACAGAGTTAGAC |
| Suggested primer 2: | AGGTTACCAGCAAATGCCAC |