| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101670 |
| Chromosome: | chromosome 3 |
| Location: | 7418389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206000 | (1 of 1) PTHR16301//PTHR16301:SF2 - IMPACT-RELATED // PROTEIN IMPACT HOMOLOG | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGATCCGTAAATTGACTCCAGTGCGCA |
| Internal bar code: | CTGGCAGTAATGAGGAAGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 98.6906 |
| LEAP-Seq n confirming: | 2638 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCCGTACTGCAACAGAT |
| Suggested primer 2: | CCAGTGAAACCTTCCACGAT |