| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101686 |
| Chromosome: | chromosome 6 |
| Location: | 5795859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGCCCGAGCAGGCGCGGCTCTCGGGG |
| Internal bar code: | TTTTCTTCTTGGGGTTACTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 98.9152 |
| LEAP-Seq n confirming: | 1003 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATTCTTCTCCCTAGTGC |
| Suggested primer 2: | AACAACACGCCGTAGGAGTC |