Insertion junction: LMJ.RY0402.101689_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.101689
Chromosome:chromosome 1
Location:6651074
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g047700 CYN40 Cyclophilin 40; (1 of 2) K05864 - peptidyl-prolyl isomerase D [EC:5.2.1.8] (PPID, CYPD) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAAAGCACGGCAGCACCCACGGCGACAC
Internal bar code:TTCGACAGTGCGGTGGGGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:622
LEAP-Seq percent confirming:78.4245
LEAP-Seq n confirming:3126
LEAP-Seq n nonconfirming:860
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGTGTGCAAAAAGGAGGG
Suggested primer 2:CAAACACCCACACACACACA