Insertion junction: LMJ.RY0402.101709_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.101709
Chromosome:chromosome 1
Location:108369
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g000750 ZFR2 zinc finger protein; (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAACATGCGCTCGGTGCGGTCCTTGAAG
Internal bar code:ACGGTCGTCTGTCTCAGGGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:289
LEAP-Seq percent confirming:99.6948
LEAP-Seq n confirming:9472
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGATCCTGTTCTGCAACG
Suggested primer 2:GTTATGAGGTGTGGGGTTGG