| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101718 |
| Chromosome: | chromosome 10 |
| Location: | 3905652 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447900 | (1 of 1) PF12239 - Protein of unknown function (DUF3605) (DUF3605) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCCGGCATCTCCGACTGCACTTGCGTT |
| Internal bar code: | GTTCGTTCCCACTTAGCTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.3647 |
| LEAP-Seq n confirming: | 3910 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGCGAAGTATACAGGGA |
| Suggested primer 2: | CGCACATGTGATGGTTAAGG |