| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101720 |
| Chromosome: | chromosome 16 |
| Location: | 5634907 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679550 | FAP277 | Flagellar Associated Protein 277; (1 of 1) K13963 - serpin B (SERPINB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCGCGGCAGCTTCAAGGAACAGCGCG |
| Internal bar code: | GGGCGCAGAATTCAACGTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 99.6694 |
| LEAP-Seq n confirming: | 1206 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTAAAGCCGTTGGACAG |
| Suggested primer 2: | CCGTACTCACTGGTCGGTTT |