| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101741 |
| Chromosome: | chromosome 12 |
| Location: | 6369509 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537500 | (1 of 1) PTHR11200//PTHR11200:SF136 - INOSITOL 5-PHOSPHATASE // PHOSPHOINOSITIDE PHOSPHATASE SAC1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCAAGTGCACATCACAACCACACATC |
| Internal bar code: | TTTCCTTGCTCTGTAGTCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 99.7353 |
| LEAP-Seq n confirming: | 6406 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCAAGTTTGGCGAGTTC |
| Suggested primer 2: | CCCTCTGTAACATCCGCATT |