Insertion junction: LMJ.RY0402.101753_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.101753
Chromosome:chromosome 11
Location:1041590
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467670 GT90F44,GT90-44 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 44 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCGCTGGGCCAGTTCCTCAACTTCCTCT
Internal bar code:GGTGGCGAAGCCTTTGAGACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:530
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GAATCATACAACATCCCCCG
Suggested primer 2:CAAAATGTGCAAAGGTGGTG