| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101765 |
| Chromosome: | chromosome 14 |
| Location: | 3009602 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628100 | SPL7 | Putative hnRNP splicing factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTGGCCCCGGCGGCCAAATGCTGCCGC |
| Internal bar code: | CACTCCGACCGAGCGGAAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 125 |
| LEAP-Seq percent confirming: | 14.8762 |
| LEAP-Seq n confirming: | 1202 |
| LEAP-Seq n nonconfirming: | 6878 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCTCCTTCACGTTTCCT |
| Suggested primer 2: | CCCAGCAAACACACACAAAC |