Insertion junction: LMJ.RY0402.101771_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.101771
Chromosome:chromosome 4
Location:1883890
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTCGCTGCGCATGTAGGCGCGCACCG
Internal bar code:ACCTCTAAGTCGAGGGCCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:291
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTTCTCCACCAACACAG
Suggested primer 2:TCTTGCACCACCACTGCTAC