| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101783 |
| Chromosome: | chromosome 3 |
| Location: | 5290178 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183650 | GDP4,GDPD4 | (1 of 3) K01126 - glycerophosphoryl diester phosphodiesterase (E3.1.4.46, glpQ, ugpQ); Glycerophosphoryl diester phosphodiesterase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGAGGGCGTCTGTGCTGGAGGCCAGG |
| Internal bar code: | TTAATTATTACGGTGATCTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 98.5143 |
| LEAP-Seq n confirming: | 2586 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTGAGAGGAAATGGCACG |
| Suggested primer 2: | CCTCGAAGGACTGCAGAAAC |