| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101795 |
| Chromosome: | chromosome 10 |
| Location: | 3898540 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447850 | YEE2 | (1 of 3) K07112 - uncharacterized protein (K07112); Integral membrane protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACCAACCAATCTTAGCCTGCGCGCCGG |
| Internal bar code: | GTTGCAGCTAGTCTCGGAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 97.541 |
| LEAP-Seq n confirming: | 2142 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGTCATTCTTCGTCAGC |
| Suggested primer 2: | GCTTGATGTGTTCTGGGGTT |