| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101833 |
| Chromosome: | chromosome 10 |
| Location: | 3959409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448550 | (1 of 3) PF14240 - YHYH protein (YHYH) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGAAACATCAGGAGGCTGGTCGTTACAC |
| Internal bar code: | TCACAGGTGGGTGCCGGCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 97.9707 |
| LEAP-Seq n confirming: | 3476 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGAGTGCGTAGGACAGA |
| Suggested primer 2: | GCGTTTTTGGGCACTGTTAT |