| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101902 |
| Chromosome: | chromosome 1 |
| Location: | 1477661 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007600 | (1 of 2) 5.5.1.6 - Chalcone isomerase / Chalcone--flavanone isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAACCAACTTCAAGTCAACACCGAACCA |
| Internal bar code: | GCGTAGTCTGGTGATTTCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 600 |
| LEAP-Seq percent confirming: | 99.4311 |
| LEAP-Seq n confirming: | 2272 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | GAGGTAGCAAGAGCGGACAC |