| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101909 |
| Chromosome: | chromosome 9 |
| Location: | 3740688 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391245 | ATG1 | (1 of 1) K08269 - serine/threonine-protein kinase ULK/ATG1 (ULK1_2_3, ATG1); Autophagy related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATCAGATTCCGTTAGGTCACGGGTGAT |
| Internal bar code: | GCCCCCAAAAGCCTATTCTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 99.1381 |
| LEAP-Seq n confirming: | 4256 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGGCTCCTGTCCGTAG |
| Suggested primer 2: | AGCGAGCTCCTGGTACGTTA |