| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.101912 |
| Chromosome: | chromosome 1 |
| Location: | 566867 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003300 | (1 of 2) PTHR30483 - LEUCINE-SPECIFIC-BINDING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGCCGCGGCAAGCATGGGAGCCTTCAT |
| Internal bar code: | TCTGATGGGAAGCGTAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1171 |
| LEAP-Seq percent confirming: | 99.463 |
| LEAP-Seq n confirming: | 1667 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGATAGCGGCTGAGTGTG |
| Suggested primer 2: | AAAATGTGCCCTACTGGTCG |