| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.101919 |
| Chromosome: | chromosome 2 |
| Location: | 7179597 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146300 | (1 of 2) PTHR10027 - CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGCATCCCGCGCCGACGGTCGTCCAG |
| Internal bar code: | ATATATGCGCTCGCCAGAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 771 |
| LEAP-Seq percent confirming: | 98.9872 |
| LEAP-Seq n confirming: | 1857 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCAGCAGTCCTTCCT |
| Suggested primer 2: | AGGCTGCAGTGATGAGAAGC |