| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.101944 |
| Chromosome: | chromosome 17 |
| Location: | 3672110 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726700 | (1 of 4) 6.2.1.1 - Acetate--CoA ligase / Acyl-activating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGAGCCAGGGGGCGCGGGGCCTGGCCG |
| Internal bar code: | CTCGCGCGTACGGCGTACGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 98.8876 |
| LEAP-Seq n confirming: | 7823 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAAACAGCCCGTTCAACC |
| Suggested primer 2: | AACACTTGGCATGGGTTAGC |