Insertion junction: LMJ.RY0402.101946_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.101946
Chromosome:chromosome 3
Location:3579918
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g168250 (1 of 6) PF06701 - Mib_herc2 (MIB_HERC2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAAGCACGAAATAGGGGAACGGATTGCT
Internal bar code:CCTGGACGCAAGCAATGTATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:750
LEAP-Seq percent confirming:92.9863
LEAP-Seq n confirming:4680
LEAP-Seq n nonconfirming:353
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGCAACCTATGACTCGCA
Suggested primer 2:AAATCAAATGCTGGGCTGTC